Hotspot

rps15-ycf1

SSC · 125485-125880 · 396 bp

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Pi 0.0135
MarkerSeek score 48.80
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
396
variable_sites
8
pi
0.0135
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 AGTCGTTGACGTTTTCCCAA GAATTGATGCAGAAGGGGGT 45.0 50.0 58.0 57.9 1136 bp 1.0 79.8
rps15-ycf1_p2 AGTCGTTGACGTTTTCCCAA TGAATTGGAATGGCGTCGAT 45.0 45.0 58.0 58.0 835 bp 0.666667 67.1
rps15-ycf1_p3 GCCTAATTGTTCGGGTCCAT TGAATTGGAATGGCGTCGAT 50.0 45.0 57.9 58.0 1301 bp 0.666667 66.6
rps15-ycf1_p4 ATGAGTCGAAGAACACCGTG TGAATTGGAATGGCGTCGAT 50.0 45.0 57.9 58.0 1117 bp 0.666667 66.6
rps15-ycf1_p5 AGCTATCCGACTCAACTCCA TGAATTGGAATGGCGTCGAT 50.0 45.0 57.9 58.0 1367 bp 0.666667 65.9