Hotspot

accD-psaI

LSC · 60909-61528 · 620 bp

Back to Datura

Pi 0.0043
MarkerSeek score 61.50
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
620
variable_sites
4
pi
0.0043
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 AAGGTTCACAAGCAGCTGAA AAGAAGCCATTGCAATTGCC 45.0 45.0 57.9 57.9 809-810 bp 1.0 79.1
accD-psaI_p2 AAGGTTCACAAGCAGCTGAA TGCAATTGCCGGAAAGACTA 45.0 45.0 57.9 57.8 799-800 bp 1.0 78.4
accD-psaI_p3 AAGGTTCACAAGCAGCTGAA CCGGAAAGACTAGGCCTACT 45.0 55.0 57.9 58.2 791-792 bp 1.0 78.2
accD-psaI_p4 AAGGTTCACAAGCAGCTGAA AGACTAGGCCTACTAACGGC 45.0 55.0 57.9 58.3 785-786 bp 1.0 77.5
accD-psaI_p5 TTAAGCTCCACGCCTTTTGA AAGAAGCCATTGCAATTGCC 45.0 45.0 57.7 57.9 716 bp 0.666667 63.8