Hotspot

atpB-rbcL

LSC · 56376-57198 · 823 bp

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Pi 0.00649
MarkerSeek score 52.50
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
823
variable_sites
8
pi
0.00649
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 GGAAACCCCAGAACCAGAAG CACCAGCTTTGAATCCAACAC 55.0 47.6 58.1 58.0 908-911 bp 1.0 72.1
atpB-rbcL_p2 GGAAACCCCAGAACCAGAAG ACCAGCTTTGAATCCAACACT 55.0 42.9 58.1 58.1 907-910 bp 1.0 71.9
atpB-rbcL_p3 GGAAACCCCAGAACCAGAAG CCTTGGTTTGGTACTCAGGAG 55.0 52.4 58.1 57.9 956-959 bp 1.0 71.7
atpB-rbcL_p4 GGAAACCCCAGAACCAGAAG CCTTGGTTTGGTACTCAGGA 55.0 50.0 58.1 56.8 956-959 bp 1.0 70.3
atpB-rbcL_p5 GGAAACCCCAGAACCAGAAG TCCTTGGTTTGGTACTCAGG 55.0 50.0 58.1 56.8 957-960 bp 1.0 70.3