Hotspot

trnM-CAU-atpE

LSC · 54260-54480 · 221 bp

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Pi 0.0181
MarkerSeek score 64.00
Primer available yes
Label trnM-CAU-atpE

Feature Metadata

label_name
trnM-CAU-atpE
feature_type
igs
region
LSC
length_bp
221
variable_sites
6
pi
0.0181
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-atpE_p1 AGTACTGCTTTCATACGGCG GGCAAATCTAGCTCTCCGAC 50.0 55.0 58.1 58.1 333 bp 1.0 79.7
trnM-CAU-atpE_p2 AGTACTGCTTTCATACGGCG GAGGCAAATCTAGCTCTCCG 50.0 55.0 58.1 57.9 335 bp 1.0 79.6
trnM-CAU-atpE_p3 AGTACTGCTTTCATACGGCG AGGCAAATCTAGCTCTCCGA 50.0 50.0 58.1 58.2 334 bp 1.0 79.1
trnM-CAU-atpE_p4 AGTACTGCTTTCATACGGCG AAGGCTGAAGGAAGGAGACA 50.0 50.0 58.1 58.3 362 bp 1.0 78.6
trnM-CAU-atpE_p5 AGTACTGCTTTCATACGGCG AGGCTGAAGGAAGGAGACAA 50.0 50.0 58.1 58.3 361 bp 1.0 78.6