Pi
0.00471
MarkerSeek score
62.40
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 758-771 bp | 1.0 | 79.8 |
| trnT-UGU-trnL-UAA_p2 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 766-779 bp | 1.0 | 79.3 |
| trnT-UGU-trnL-UAA_p3 | AAGAATAGGGAAAAGCCGGC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.2 | 58.0 | 814-827 bp | 1.0 | 79.0 |
| trnT-UGU-trnL-UAA_p4 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 790-803 bp | 1.0 | 78.9 |
| trnT-UGU-trnL-UAA_p5 | AAGAATAGGGAAAAGCCGGC | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 58.2 | 58.1 | 822-835 bp | 1.0 | 78.4 |