Hotspot

ycf3-trnS-GGA

LSC · 46090-46618 · 529 bp

Back to Datura

Pi 0.0101
MarkerSeek score 60.20
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
529
variable_sites
8
pi
0.0101
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TTCTCCTGAAGTTGTCGGAA GCGAAGGTACGGAAAGAGAG 45.0 55.0 56.4 58.1 721-731 bp 1.0 67.5
ycf3-trnS-GGA_p2 TTTCTCCTGAAGTTGTCGGA GCGAAGGTACGGAAAGAGAG 45.0 55.0 56.4 58.1 722-732 bp 1.0 67.5
ycf3-trnS-GGA_p3 TTCTCCTGAAGTTGTCGGAA CCCTCGGTAAACAAAAGCCT 45.0 50.0 56.4 58.1 692-702 bp 1.0 67.5
ycf3-trnS-GGA_p4 TTTCTCCTGAAGTTGTCGGA CCCTCGGTAAACAAAAGCCT 45.0 50.0 56.4 58.1 693-703 bp 1.0 67.5
ycf3-trnS-GGA_p5 TTCTCCTGAAGTTGTCGGAA ACGGAAAGAGAGGGATTCGA 45.0 50.0 56.4 58.2 713-723 bp 1.0 67.0