Hotspot

rps16-trnQ-UUG

LSC · 6195-7378 · 1184 bp

Back to Datura

Pi 0.00736
MarkerSeek score 53.80
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1184
variable_sites
13
pi
0.00736
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1250-1257 bp 1.0 79.9
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 1254-1261 bp 1.0 75.1
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA TAAGGCAACGGGTTTTGGTC 45.0 50.0 58.1 58.7 1280-1287 bp 1.0 75.0
rps16-trnQ-UUG_p4 CGTTGCTTTCTACCACATCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.2 58.1 1242-1249 bp 1.0 74.7
rps16-trnQ-UUG_p5 AGTCGCACGTTGCTTTCTAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.9 58.1 1249-1256 bp 1.0 74.1