Hotspot

rpl16

LSC · 77811-79290 · 1480 bp

Back to Dasypyrum

Pi 0.00542

MarkerSeek score 52.50

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1480
variable_sites: 8
pi: 0.00542
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCCAGAAAGAAAAACCGGGC	CTGCAAAAGAAGAAATTCTACCTCT	50.0	36.0	58.7	57.6	1604-1605 bp	1.0	40.5
rpl16_p2	TCCAGAAAGAAAAACCGGGC	TGCAAAAGAAGAAATTCTACCTCT	50.0	33.3	58.7	56.5	1603-1604 bp	1.0	40.5
rpl16_p3	TCCAGAAAGAAAAACCGGGC	CTGCAAAAGAAGAAATTCTACCTC	50.0	37.5	58.7	56.3	1604-1605 bp	1.0	40.5
rpl16_p4	CCAGAAAGAAAAACCGGGCA	CTGCAAAAGAAGAAATTCTACCTCT	50.0	36.0	59.0	57.6	1603-1604 bp	1.0	40.5
rpl16_p5	CCAGAAAGAAAAACCGGGCA	TGCAAAAGAAGAAATTCTACCTCT	50.0	33.3	59.0	56.5	1602-1603 bp	1.0	40.5