Hotspot

psbB

LSC · 68980-70506 · 1527 bp

Back to Dasypyrum

Pi 0.00393

MarkerSeek score 61.00

Primer available yes

Label psbB

Feature Metadata

label_name: psbB
feature_type: gene
region: LSC
length_bp: 1527
variable_sites: 6
pi: 0.00393
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB_p1	TCTGACACAGAATCCCCTAGA	AGAGTCAAGGATGGGAGATGT	47.6	47.6	57.2	57.9	1712 bp	1.0	57.2
psbB_p2	CGCGCTTTCTGACACAGAA	AGAGTCAAGGATGGGAGATGT	52.6	47.6	58.8	57.9	1719 bp	1.0	57.1
psbB_p3	GACACAGAATCCCCTAGAAGG	AGAGTCAAGGATGGGAGATGT	52.4	47.6	57.2	57.9	1709 bp	1.0	56.6
psbB_p4	TCTGACACAGAATCCCCTAGAA	AGAGTCAAGGATGGGAGATGT	45.5	47.6	57.9	57.9	1712 bp	1.0	54.8
psbB_p5	TTCTGACACAGAATCCCCTAGA	AGAGTCAAGGATGGGAGATGT	45.5	47.6	57.9	57.9	1713 bp	1.0	54.8