Hotspot

rpl32-trnL-UAG

SSC · 104895-105596 · 702 bp

Back to Dasypyrum

Pi 0.0415

MarkerSeek score 83.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 702
variable_sites: 29
pi: 0.0415
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TTCCAGCGGTAATGAGCATC	TTCGAACCGAGATGCTTGAG	50.0	50.0	58.0	58.0	841-844 bp	1.0	82.7
rpl32-trnL-UAG_p2	TTCCAGCGGTAATGAGCATC	GGATTCGAACCGAGATGCTT	50.0	50.0	58.0	58.1	844-847 bp	1.0	82.3
rpl32-trnL-UAG_p3	AAGGGTTTTTCTGGGCAACA	TTCGAACCGAGATGCTTGAG	45.0	50.0	58.1	58.0	813-816 bp	1.0	82.2
rpl32-trnL-UAG_p4	AGGGTTTTTCTGGGCAACAA	TTCGAACCGAGATGCTTGAG	45.0	50.0	58.1	58.0	812-815 bp	1.0	82.2
rpl32-trnL-UAG_p5	TTCCAGCGGTAATGAGCATC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	814-817 bp	1.0	81.9