Hotspot

atpB-rbcL

LSC · 53657-54438 · 782 bp

Back to Dasypyrum

Pi 0.00639

MarkerSeek score 54.30

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 782
variable_sites: 5
pi: 0.00639
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TTCAATTGTGGAAACCCCGG	GCTTGAAATCCAACACCTGC	50.0	50.0	58.7	57.9	874-878 bp	1.0	74.6
atpB-rbcL_p2	CTTCAATTGTGGAAACCCCG	GCTTGAAATCCAACACCTGC	50.0	50.0	57.3	57.9	875-879 bp	1.0	74.1
atpB-rbcL_p3	TCAATTGTGGAAACCCCGG	GCTTGAAATCCAACACCTGC	52.6	50.0	58.0	57.9	873-877 bp	1.0	71.5
atpB-rbcL_p4	AGCACGGGTCCAATAATTTGA	GCTTGAAATCCAACACCTGC	42.9	50.0	57.9	57.9	918-922 bp	1.0	71.2
atpB-rbcL_p5	TTCAATTGTGGAAACCCCGG	CCAGCTTGAAATCCAACACC	50.0	50.0	58.7	57.3	877-881 bp	1.0	69.3