Hotspot

trnC-GCA-rpoB

LSC · 18192-19348 · 1157 bp

Back to Dasypyrum

Pi 0.00437
MarkerSeek score 62.30
Primer available yes
Label trnC-GCA-rpoB

Feature Metadata

label_name
trnC-GCA-rpoB
feature_type
igs
region
LSC
length_bp
1157
variable_sites
5
pi
0.00437
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-rpoB_p1 AAAGGATTTGCAGTCCCCTG ATTCCCTCGTTTCCATTCCG 50.0 50.0 58.1 58.0 1221-1226 bp 1.0 80.2
trnC-GCA-rpoB_p2 AAAGGATTTGCAGTCCCCTG TGTGGACATTCCCTCGTTTC 50.0 50.0 58.1 57.8 1228-1233 bp 1.0 79.1
trnC-GCA-rpoB_p3 AAAGGATTTGCAGTCCCCTG CCGGGTATTGTGGACATTCC 50.0 55.0 58.1 58.3 1236-1241 bp 1.0 77.9
trnC-GCA-rpoB_p4 AAAGGATTTGCAGTCCCCTG CGGGTATTGTGGACATTCCC 50.0 55.0 58.1 58.3 1235-1240 bp 1.0 77.9
trnC-GCA-rpoB_p5 AAAGGATTTGCAGTCCCCTG AAGTTCTTCTGCCAAGGCTT 50.0 45.0 58.1 57.6 1300-1305 bp 1.0 77.5