Hotspot

psbM-petN

LSC · 16804-17541 · 738 bp

Back to Dasypyrum

Pi 0.00695

MarkerSeek score 65.20

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 738
variable_sites: 5
pi: 0.00695
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TGCTACTGCACTGTTCATTCT	AGTATGGGGAAGGAGTGGAC	42.9	55.0	57.6	58.1	824-842 bp	1.0	70.1
psbM-petN_p2	TGCTACTGCACTGTTCATTCT	GTATGGGGAAGGAGTGGACT	42.9	55.0	57.6	58.1	823-841 bp	1.0	70.1
psbM-petN_p3	TGCTACTGCACTGTTCATTCT	TAGTAAGTCTCGCTTGGGCT	42.9	50.0	57.6	58.2	882-900 bp	1.0	69.7
psbM-petN_p4	TGCTACTGCACTGTTCATTCT	TATGGGGAAGGAGTGGACTC	42.9	55.0	57.6	57.8	822-840 bp	1.0	69.6
psbM-petN_p5	TGCTACTGCACTGTTCATTCT	GGGGAAGGAGTGGACTCTAG	42.9	60.0	57.6	58.2	819-837 bp	1.0	69.3