Hotspot

trnD-GUC-psbM

LSC · 15907-16698 · 792 bp

Back to Dasypyrum

Pi 0.00505

MarkerSeek score 61.70

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 792
variable_sites: 4
pi: 0.00505
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	TTCAATTGGTCAGAGCACCG	AGAATGAACAGTGCAGTAGC	50.0	45.0	58.5	55.8	903-916 bp	1.0	58.7
trnD-GUC-psbM_p2	TTCAATTGGTCAGAGCACCG	AGGAACTAGAATGAACAGTGCA	50.0	40.9	58.5	57.4	910-923 bp	1.0	55.5
trnD-GUC-psbM_p3	GTTCAATTGGTCAGAGCACC	AGAATGAACAGTGCAGTAGC	50.0	45.0	57.0	55.8	904-917 bp	1.0	54.5
trnD-GUC-psbM_p4	CGCGGGGATTGTAGTTCAA	AGAATGAACAGTGCAGTAGC	52.6	45.0	57.9	55.8	917-930 bp	1.0	53.3
trnD-GUC-psbM_p5	TTCAATTGGTCAGAGCACCG	GGAACTAGAATGAACAGTGCAG	50.0	45.5	58.5	57.1	909-922 bp	1.0	53.0