Hotspot

trnS-GCU-psbD

LSC · 7624-8603 · 980 bp

Back to Dasypyrum

Pi 0.00617

MarkerSeek score 63.00

Primer available yes

Label trnS-GCU-psbD

Feature Metadata

label_name: trnS-GCU-psbD
feature_type: igs
region: LSC
length_bp: 980
variable_sites: 6
pi: 0.00617
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psbD_p1	ATTAGCAATCCGCCGCTTTA	GAGCAATAGGCCAGACCATC	45.0	55.0	58.0	58.1	1127-1134 bp	1.0	79.7
trnS-GCU-psbD_p2	ATTAGCAATCCGCCGCTTTA	ACAAAAACGAAACGGTCCCT	45.0	45.0	58.0	58.2	1105-1112 bp	1.0	78.7
trnS-GCU-psbD_p3	ATTAGCAATCCGCCGCTTTA	CCCTTCGTAACCAGTCATCC	45.0	55.0	58.0	57.7	1089-1096 bp	1.0	78.2
trnS-GCU-psbD_p4	GAGCGGAAAGAGAGGGATTC	GAGCAATAGGCCAGACCATC	55.0	55.0	57.8	58.1	1178-1185 bp	1.0	78.1
trnS-GCU-psbD_p5	ATTAGCAATCCGCCGCTTTA	AGGCCAGACCATCCTACAAA	45.0	50.0	58.0	58.3	1120-1127 bp	1.0	78.0