Hotspot

psbK-psbI

LSC · 6902-7307 · 406 bp

Back to Dasypyrum

Pi 0.0226

MarkerSeek score 69.30

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 406
variable_sites: 9
pi: 0.0226
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TTTTTACTCCTCGCGTCCAG	50.0	50.0	57.9	57.9	543-550 bp	1.0	81.3
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TTCCGTCCTGGGTCATTAGA	50.0	50.0	57.9	57.8	521-528 bp	1.0	80.1
psbK-psbI_p3	TGGCAAGCTGCTGTAAGTTT	TTTTTACTCCTCGCGTCCAG	45.0	50.0	58.3	57.9	540-547 bp	1.0	79.3
psbK-psbI_p4	TTGGCAAGCTGCTGTAAGTT	TTTTTACTCCTCGCGTCCAG	45.0	50.0	58.3	57.9	541-548 bp	1.0	79.3
psbK-psbI_p5	TTTGGCAAGCTGCTGTAAGT	TTTTTACTCCTCGCGTCCAG	45.0	50.0	58.3	57.9	542-549 bp	1.0	79.3