Hotspot

ycf37

Genome · 6700-7212 · 513 bp

Back to Dasyclonium

Pi 0.191

MarkerSeek score 76.90

Primer available yes

Label ycf37

Feature Metadata

label_name: ycf37
feature_type: gene
region: Genome
length_bp: 513
variable_sites: 175
pi: 0.191
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf37_p1	CCACTTTTAGTTCTCATTCTTGCT	ATGCAGTAGGATGGGTACGT	37.5	50.0	57.4	58.2	1201 bp	0.5	23.9
ycf37_p2	TGCATTCATAATTGTTTGTCCACT	ATGCAGTAGGATGGGTACGT	33.3	50.0	57.7	58.2	1220 bp	0.25	13.6
ycf37_p3	TGCATTCATAATTGTTTGTCCACT	TGTGATGCAGTAGGATGGGT	33.3	50.0	57.7	58.4	1224 bp	0.25	11.9
ycf37_p4	TCCACTTTTAGTTCTCATTCTTGC	ATGCAGTAGGATGGGTACGT	37.5	50.0	57.4	58.2	1202 bp	0.25	11.8
ycf37_p5	TGCATTCATAATTGTTTGTCCACT	TGCAGTAGGATGGGTACGTA	33.3	50.0	57.7	57.6	1219 bp	0.25	11.7