Hotspot

syfB

Genome · 158561-160549 · 1989 bp

Back to Dasyclonium

Pi 0.176

MarkerSeek score 78.80

Primer available yes

Label syfB

Feature Metadata

label_name: syfB
feature_type: gene
region: Genome
length_bp: 1989
variable_sites: 616
pi: 0.176
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
syfB_p1	GAGGATCGTGCCACAGAAAT	CGAATCCTCCTACCCCAGAT	50.0	55.0	58.0	58.0	2876-2889 bp	1.0	88.8
syfB_p2	AGGAAAGTCCGGGCTCTATT	CGAATCCTCCTACCCCAGAT	50.0	55.0	58.1	58.0	2937-2950 bp	1.0	88.1
syfB_p3	GAGGATCGTGCCACAGAAAT	GGTGTAGCCAAGTGGTAAGG	50.0	55.0	58.0	57.9	2927-2940 bp	1.0	88.0
syfB_p4	GAGGATCGTGCCACAGAAAT	CTAACTCGATCTCGCGATGG	50.0	55.0	58.0	58.0	2676-2677 bp	0.75	78.1
syfB_p5	GTAAGGGTGCAATGGTACGT	CGAATCCTCCTACCCCAGAT	50.0	55.0	57.9	58.0	2846-2847 bp	0.5	61.2