Hotspot

ssrA

Genome · 55012-55448 · 437 bp

Back to Dasyclonium

Pi 0.0816

MarkerSeek score 80.40

Primer available yes

Label ssrA

Feature Metadata

label_name: ssrA
feature_type: gene
region: Genome
length_bp: 437
variable_sites: 65
pi: 0.0816
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ssrA_p1	ACTTTGGTGGTCGAAGGTTC	TCAGACATAATAACTTGGCGCT	50.0	40.9	58.0	57.9	1314-1325 bp	0.5	44.9
ssrA_p2	GACTTTGGTGGTCGAAGGTT	TCAGACATAATAACTTGGCGCT	50.0	40.9	58.0	57.9	1315-1326 bp	0.5	44.9
ssrA_p3	ACTTTGGTGGTCGAAGGTTC	ACAATTCTTTGGCGTTTCACT	50.0	38.1	58.0	56.9	772-783 bp	0.5	44.3
ssrA_p4	GACTTTGGTGGTCGAAGGTT	ACAATTCTTTGGCGTTTCACT	50.0	38.1	58.0	56.9	773-784 bp	0.5	44.3
ssrA_p5	CTTTGGTGGTCGAAGGTTCA	ACAATTCTTTGGCGTTTCACT	50.0	38.1	58.0	56.9	771-782 bp	0.5	44.3