Hotspot

rps1

Genome · 157333-158127 · 795 bp

Back to Dasyclonium

Pi 0.152

MarkerSeek score 80.20

Primer available yes

Label rps1

Feature Metadata

label_name: rps1
feature_type: gene
region: Genome
length_bp: 795
variable_sites: 216
pi: 0.152
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps1_p1	CGCGTATGATGGAGTCACAA	ATTTCTGTGGCACGATCCTC	50.0	50.0	58.1	58.0	1268-1283 bp	1.0	88.1
rps1_p2	GGAGTCACAATCCATTGCCT	ATTTCTGTGGCACGATCCTC	50.0	50.0	57.9	58.0	1258-1273 bp	1.0	87.6
rps1_p3	TTATTGAGGGCGAATGACGG	ATTTCTGTGGCACGATCCTC	50.0	50.0	58.1	58.0	1298-1307 bp	0.75	77.9
rps1_p4	TTATTGAGGGCGAATGACGG	ACGTACCATTGCACCCTTAC	50.0	50.0	58.1	57.9	1336-1345 bp	0.75	77.3
rps1_p5	TTATTGAGGGCGAATGACGG	AATAGAGCCCGGACTTTCCT	50.0	50.0	58.1	58.1	1237-1246 bp	0.75	77.2