Hotspot

dnaB

Genome · 148730-150556 · 1827 bp

Back to Dasyclonium

Pi 0.172

MarkerSeek score 78.90

Primer available yes

Label dnaB

Feature Metadata

label_name: dnaB
feature_type: gene
region: Genome
length_bp: 1827
variable_sites: 558
pi: 0.172
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
dnaB_p1	TCAGTCCACTGCTCTACCAA	TGCAGAAATAGCAACAAACA	50.0	35.0	58.0	54.0	2325 bp	0.25	18.0
dnaB_p2	TCCACTGCTCTACCAACTGA	TGCAGAAATAGCAACAAACA	50.0	35.0	58.0	54.0	2321 bp	0.25	18.0
dnaB_p3	TTCAGTCCACTGCTCTACCA	TGCAGAAATAGCAACAAACA	50.0	35.0	58.0	54.0	2326 bp	0.25	18.0
dnaB_p4	ACTGAGCTATCCCGGCTAAT	TGCAGAAATAGCAACAAACA	50.0	35.0	58.0	54.0	2306 bp	0.25	17.9
dnaB_p5	AGTCCACTGCTCTACCAACT	TGCAGAAATAGCAACAAACA	50.0	35.0	58.0	54.0	2323 bp	0.25	17.9