Hotspot

orf5-cemA

Genome · 66874-67598 · 725 bp

Back to Dasyclonium

Pi 0.0952

MarkerSeek score 84.30

Primer available yes

Label orf5-cemA

Feature Metadata

label_name: orf5-cemA
feature_type: igs
region: Genome
length_bp: 725
variable_sites: 123
pi: 0.0952
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf5-cemA_p1	TTTGCTTTCCATTCTGGCTT	TCCTTCAGCTGTTGCAACAT	40.0	45.0	56.1	58.0	1072-1073 bp	0.5	46.2
orf5-cemA_p2	TTTTGCTTTCCATTCTGGCT	TCCTTCAGCTGTTGCAACAT	40.0	45.0	56.1	58.0	1073-1074 bp	0.5	46.2
orf5-cemA_p3	TTTTGCTTTCCATTCTGGCTT	TCCTTCAGCTGTTGCAACAT	38.1	45.0	56.9	58.0	1073-1074 bp	0.5	44.4
orf5-cemA_p4	TTTTTGCTTTCCATTCTGGCT	TCCTTCAGCTGTTGCAACAT	38.1	45.0	56.9	58.0	1074-1075 bp	0.5	44.4
orf5-cemA_p5	GTTTTTGCTTTCCATTCTGGC	TCCTTCAGCTGTTGCAACAT	42.9	45.0	56.8	58.0	1075-1076 bp	0.5	44.1