Hotspot

psaC-ycf22

Genome · 46925-47899 · 975 bp

Back to Dasyclonium

Pi 0.204
MarkerSeek score 80.30
Primer available yes
Label psaC-ycf22

Feature Metadata

label_name
psaC-ycf22
feature_type
igs
region
Genome
length_bp
975
variable_sites
347
pi
0.204
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ycf22_p1 AAATTGCTTCAGCGCCTAGA CGAGGATCATCAAACCTCTGA 45.0 47.6 57.9 57.2 1639-1643 bp 0.5 48.7
psaC-ycf22_p2 AATTGCTTCAGCGCCTAGAA CGAGGATCATCAAACCTCTGA 45.0 47.6 57.9 57.2 1638-1642 bp 0.5 48.7
psaC-ycf22_p3 AATTGCTTCAGCGCCTAGAA TCGAGGATCATCAAACCTCTG 45.0 47.6 57.9 57.2 1639-1643 bp 0.5 48.7
psaC-ycf22_p4 CAGGTCAAATTGCTTCAGCG CGAGGATCATCAAACCTCTGA 50.0 47.6 58.0 57.2 1645 bp 0.25 35.3
psaC-ycf22_p5 CAGGTCAAATTGCTTCAGCG TCGAGGATCATCAAACCTCTG 50.0 47.6 58.0 57.2 1646 bp 0.25 35.3