Hotspot

rps3

LSC · 82461-83117 · 657 bp

Back to Dasiphora

Pi 0.00558
MarkerSeek score 49.90
Primer available yes
Label rps3

Feature Metadata

label_name
rps3
feature_type
gene
region
LSC
length_bp
657
variable_sites
5
pi
0.00558
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps3_p1 TCACTAAATATAAGCCAAGTAAATGCT TGATATATATAGTAGTGGGGGACT 29.6 37.5 57.4 54.9 710 bp 0.666667 27.8
rps3_p2 TCACTAAATATAAGCCAAGTAAATGC TGATATATATAGTAGTGGGGGACT 30.8 37.5 56.2 54.9 710 bp 0.666667 27.8
rps3_p3 CACTAAATATAAGCCAAGTAAATGCT TGATATATATAGTAGTGGGGGACT 30.8 37.5 56.2 54.9 709 bp 0.666667 27.8
rps3_p4 TCACTAAATATAAGCCAAGTAAATGCT TGATATATATAGTAGTGGGGGAC 29.6 39.1 57.4 53.5 710 bp 0.666667 27.8
rps3_p5 TCACTAAATATAAGCCAAGTAAATGCT ATGATATATATAGTAGTGGGGGACT 29.6 36.0 57.4 55.5 711 bp 0.666667 27.8