Hotspot

rpl32-trnL-UAG

SSC · 112340-113002 · 663 bp

Back to Dasiphora

Pi 0.0131

MarkerSeek score 54.90

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 663
variable_sites: 13
pi: 0.0131
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGCAGCATTGAAAGCTTTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.8	58.0	775-810 bp	1.0	80.0
rpl32-trnL-UAG_p2	GGGCAGCATTGAAAGCTTTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.8	58.0	776-811 bp	1.0	80.0
rpl32-trnL-UAG_p3	GGGCAGCATTGAAAGCTTTT	ATCCACACAAAAGAGCTGCA	45.0	45.0	57.8	58.0	1372-1407 bp	1.0	79.5
rpl32-trnL-UAG_p4	GGGCAGCATTGAAAGCTTTT	TCCACACAAAAGAGCTGCAT	45.0	45.0	57.8	58.0	1371-1406 bp	1.0	79.5
rpl32-trnL-UAG_p5	GGGCAGCATTGAAAGCTTTT	ATAACACTTGGCGCCGTTAT	45.0	45.0	57.8	58.0	1232-1267 bp	1.0	79.4