Hotspot

rps3-rpl22

LSC · 83118-83250 · 133 bp

Back to Dasiphora

Pi 0.0423

MarkerSeek score 66.70

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 133
variable_sites: 8
pi: 0.0423
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	ATACGTGCAATTCCCTCGAC	ACAGAAGTAGCCGCTTCAAG	50.0	50.0	58.1	57.9	678-708 bp	1.0	83.9
rps3-rpl22_p2	ATACGTGCAATTCCCTCGAC	CCGACTCTAAATTTCCGGGG	50.0	55.0	58.1	58.1	865-878 bp	1.0	83.1
rps3-rpl22_p3	ATTGGGGTGGCTATAAGGGT	CCGACTCTAAATTTCCGGGG	50.0	55.0	58.1	58.1	1052-1065 bp	1.0	82.4
rps3-rpl22_p4	CTTTTGTACCTGCCTGCTCA	CCGACTCTAAATTTCCGGGG	50.0	55.0	58.1	58.1	1152-1165 bp	1.0	81.9
rps3-rpl22_p5	TTTTGTACCTGCCTGCTCAG	CCGACTCTAAATTTCCGGGG	50.0	55.0	58.1	58.1	1151-1164 bp	1.0	81.9