Hotspot

psaA-ycf3

LSC · 41610-42379 · 770 bp

Back to Dasiphora

Pi 0.00791

MarkerSeek score 65.10

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 770
variable_sites: 9
pi: 0.00791
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TTACGGGATCCCTATCGACC	GCTATAGCGCTTACTCCTGG	55.0	55.0	58.1	57.6	889-982 bp	1.0	78.2
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	GCTATAGCGCTTACTCCTGG	50.0	55.0	57.9	57.6	911-1004 bp	1.0	77.9
psaA-ycf3_p3	TTACGGGATCCCTATCGACC	GGAGGCTTGGTTCGATCAAG	55.0	55.0	58.1	58.6	929-1022 bp	1.0	76.0
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	GGAGGCTTGGTTCGATCAAG	50.0	55.0	57.9	58.6	951-1044 bp	1.0	75.7
psaA-ycf3_p5	TTACGGGATCCCTATCGACC	TGGAAACAAGCTATAGCGCT	55.0	45.0	58.1	57.3	898-991 bp	1.0	75.5