Hotspot

psbK-psbI

LSC · 7557-7787 · 231 bp

Back to Dasiphora

Pi 0.0145

MarkerSeek score 61.90

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 231
variable_sites: 5
pi: 0.0145
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TCTTCACGTCCAGGATTACG	50.0	50.0	57.9	57.1	367-368 bp	1.0	73.6
psbK-psbI_p2	CAAATTGCCCGAAGCTTACG	TCTTCACGTCCAGGATTACG	50.0	50.0	58.1	57.1	452-453 bp	1.0	72.9
psbK-psbI_p3	TGGCAAGCTGCTGTAAGTTT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.3	57.1	364-365 bp	1.0	71.6
psbK-psbI_p4	TTGGCAAGCTGCTGTAAGTT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.3	57.1	365-366 bp	1.0	71.6
psbK-psbI_p5	CCAAATTGCCCGAAGCTTAC	TCTTCACGTCCAGGATTACG	50.0	50.0	57.7	57.1	453-454 bp	1.0	71.5