Hotspot

rps16-trnQ-UUG

LSC · 6046-6870 · 825 bp

Back to Dasiphora

Pi 0.00971
MarkerSeek score 56.30
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
825
variable_sites
12
pi
0.00971
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CAGCGGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 914-943 bp 1.0 81.2
rps16-trnQ-UUG_p2 GCGGATCATGTCCTTCAAGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 912-941 bp 1.0 81.2
rps16-trnQ-UUG_p3 GATGTAAGACTCCACAGCGG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.1 58.1 928-957 bp 1.0 80.9
rps16-trnQ-UUG_p4 GGATGTAAGACTCCACAGCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.1 58.1 929-958 bp 1.0 80.9
rps16-trnQ-UUG_p5 TAAGACTCCACAGCGGATCA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 924-953 bp 1.0 80.2