Hotspot

trnK-UUU-rps16

LSC · 4262-4923 · 662 bp

Back to Dasiphora

Pi 0.0141
MarkerSeek score 56.90
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
662
variable_sites
14
pi
0.0141
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT ACTTCACCTTAATCAACAAACA 50.0 31.8 58.2 54.1 730-830 bp 1.0 42.3
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT ACTTCACCTTAATCAACAAACA 50.0 31.8 58.2 54.1 729-829 bp 1.0 42.3
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT ACTTCACCTTAATCAACAAACAA 50.0 30.4 58.2 54.9 730-830 bp 1.0 42.3
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT AACTTCACCTTAATCAACAAACA 50.0 30.4 58.2 54.9 730-830 bp 1.0 42.3
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT ACTTCACCTTAATCAACAAACAA 50.0 30.4 58.2 54.9 729-829 bp 1.0 42.3