Hotspot

ycf1

SSC · 129124-134700 · 5577 bp

Back to Daphniphyllum

Pi 0.00436

MarkerSeek score 55.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5577
variable_sites: 50
pi: 0.00436
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CACTAACATTTTCCGTTCCTGT	TCACAATTCAGCAAGATCAAGA	40.9	36.4	56.9	56.0	216 bp	1.0	41.9
ycf1_p2	CACTAACATTTTCCGTTCCTGT	AGAAAGGCCTTTTTCATTTCACA	40.9	34.8	56.9	57.4	234 bp	1.0	41.7
ycf1_p3	TCACTAACATTTTCCGTTCCTGT	AGAAAGGCCTTTTTCATTTCACA	39.1	34.8	58.3	57.4	235 bp	1.0	41.7
ycf1_p4	TCACTAACATTTTCCGTTCCTG	AGAAAGGCCTTTTTCATTTCACA	40.9	34.8	56.6	57.4	235 bp	1.0	41.7
ycf1_p5	CACTAACATTTTCCGTTCCTGT	AGAAAGGCCTTTTTCATTTCACAA	40.9	33.3	56.9	58.0	234 bp	1.0	41.7