Hotspot

rpoC2

LSC · 17349-21527 · 4179 bp

Back to Daphniphyllum

Pi 0.00144

MarkerSeek score 55.00

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4179
variable_sites: 13
pi: 0.00144
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	CCTGAATCTGAATGGGGCAT	TCTCTTTCTGTCGAACGAAGA	50.0	42.9	57.6	56.7	284 bp	1.0	59.5
rpoC2_p2	CCTGAATCTGAATGGGGCAT	ACCTCTCAGTGACTGATGATCA	50.0	45.5	57.6	58.3	253 bp	1.0	59.3
rpoC2_p3	CCTGAATCTGAATGGGGCAT	CCTCTCAGTGACTGATGATCA	50.0	47.6	57.6	56.6	252 bp	1.0	58.1
rpoC2_p4	CCTGAATCTGAATGGGGCAT	ACCTCTCAGTGACTGATGATC	50.0	47.6	57.6	56.6	253 bp	1.0	58.0
rpoC2_p5	CCTGAATCTGAATGGGGCAT	AACCTCTCAGTGACTGATGA	50.0	45.0	57.6	55.5	254 bp	1.0	57.6