Hotspot

ndhD-psaC

SSC · 122334-122457 · 124 bp

Back to Daphniphyllum

Pi 0.025

MarkerSeek score 57.60

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 124
variable_sites: 6
pi: 0.025
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AAAATTAAGGAACCCGCGGA	GCATGGGGCTAGCTTATTGA	45.0	50.0	57.8	57.7	222-228 bp	1.0	80.4
ndhD-psaC_p2	AAATTAAGGAACCCGCGGAT	GCATGGGGCTAGCTTATTGA	45.0	50.0	57.6	57.7	221-227 bp	1.0	78.6
ndhD-psaC_p3	GAAAATTAAGGAACCCGCGG	GCATGGGGCTAGCTTATTGA	50.0	50.0	57.4	57.7	223-229 bp	1.0	77.6
ndhD-psaC_p4	AAAATTAAGGAACCCGCGGA	ATGAAACAACTCGCAGCATG	45.0	45.0	57.8	57.4	237-243 bp	1.0	77.4
ndhD-psaC_p5	AAAATTAAGGAACCCGCGGA	CATGAAACAACTCGCAGCAT	45.0	45.0	57.8	57.4	238-244 bp	1.0	77.4