Hotspot

rpl32-trnL-UAG

SSC · 118194-119401 · 1208 bp

Back to Daphniphyllum

Pi 0.00733
MarkerSeek score 46.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1208
variable_sites
17
pi
0.00733
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
46.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TTTGTGCCGACAAATACAAA AGCGTGTCTACCGATTTCAC 35.0 50.0 54.3 58.0 1246-1280 bp 1.0 53.1
rpl32-trnL-UAG_p2 TTTGTGCCGACAAATACAAA CAGCGTGTCTACCGATTTCA 35.0 50.0 54.3 58.0 1247-1281 bp 1.0 53.1
rpl32-trnL-UAG_p3 TTTGTGCCGACAAATACAAA TCCTAAGAGCAGCGTGTCTA 35.0 50.0 54.3 57.9 1256-1290 bp 1.0 52.1
rpl32-trnL-UAG_p4 TTTGTGCCGACAAATACAAA CCTAAGAGCAGCGTGTCTAC 35.0 55.0 54.3 57.8 1255-1289 bp 1.0 51.6
rpl32-trnL-UAG_p5 TTTGTGCCGACAAATACAAA CTAAGAGCAGCGTGTCTACC 35.0 55.0 54.3 57.8 1254-1288 bp 1.0 51.6