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psbE-petL

LSC · 68507-69778 · 1272 bp

Back to Daphniphyllum

Pi 0.00723

MarkerSeek score 50.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1272
variable_sites: 21
pi: 0.00723
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GCCGCTAGTAGAAAACCGAA	50.0	50.0	57.9	58.0	1416-1419 bp	1.0	80.8
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AAGCCGCTAGTAGAAAACCG	50.0	50.0	57.9	58.0	1418-1421 bp	1.0	80.8
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	GCCGCTAGTAGAAAACCGAA	50.0	50.0	57.8	58.0	1339-1342 bp	1.0	80.1
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	AAGCCGCTAGTAGAAAACCG	50.0	50.0	57.8	58.0	1341-1344 bp	1.0	80.0
psbE-petL_p5	ACGAATAACCAACCCGCAAT	GCCGCTAGTAGAAAACCGAA	45.0	50.0	58.2	58.0	1425-1428 bp	1.0	79.9