Hotspot

trnF-GAA-ndhJ

LSC · 51297-52030 · 734 bp

Back to Daphniphyllum

Pi 0.0054

MarkerSeek score 55.10

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 734
variable_sites: 7
pi: 0.0054
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATCCACGCCTGAAACGTATC	55.0	50.0	58.0	58.1	726-908 bp	1.0	84.2
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATCCACGCCTGAAACGTATC	50.0	50.0	58.1	58.1	690-872 bp	1.0	83.8
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	ATCCACGCCTGAAACGTATC	55.0	50.0	57.9	58.1	714-896 bp	1.0	83.1
trnF-GAA-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	GAAAGTTGGATAGGGTGGCC	55.0	55.0	58.0	58.2	697-879 bp	1.0	82.8
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	CCACGCCTGAAACGTATCTT	55.0	50.0	58.0	58.3	724-906 bp	1.0	82.4