Hotspot

trnS-GCU-trnG-GCC

LSC · 8845-9582 · 738 bp

Back to Daphniphyllum

Pi 0.00462
MarkerSeek score 56.10
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
738
variable_sites
7
pi
0.00462
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 799-817 bp 1.0 53.6
trnS-GCU-trnG-GCC_p2 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 798-816 bp 1.0 53.6
trnS-GCU-trnG-GCC_p3 CAATCCGACGCTTTAGTCCA AGAACGAATCACACTTTTACCAC 50.0 39.1 58.0 57.3 802-820 bp 1.0 52.5
trnS-GCU-trnG-GCC_p4 CAATCCGACGCTTTAGTCCA GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 801-819 bp 1.0 52.5
trnS-GCU-trnG-GCC_p5 AATCCGACGCTTTAGTCCAC GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 800-818 bp 1.0 52.5