Hotspot

rpl16

LSC · 82009-83492 · 1484 bp

Back to Daphne

Pi 0.0154
MarkerSeek score 66.70
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1484
variable_sites
111
pi
0.0154
unique_haplotype_count
19
primer_available
yes
alignment_reliability
0.98
markerseek_score
66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 AATAGGATCCCTCCCCATCG GCCGGGAAGAATCGAAGAAT 55.0 50.0 58.0 58.0 2555-2726 bp 0.947368 86.1
rpl16_p2 AATAGGATCCCTCCCCATCG ATTCCGAGGGTCTCCAAGAA 55.0 50.0 58.0 58.0 2733-2904 bp 0.947368 86.0
rpl16_p3 ATTTTTGGGCTCCGCTGTTA TACAAACCATTGGGGCGAAA 45.0 45.0 58.1 58.0 1752-1922 bp 0.789474 79.4
rpl16_p4 TTTTTGGGCTCCGCTGTTAT TACAAACCATTGGGGCGAAA 45.0 45.0 58.1 58.0 1751-1921 bp 0.789474 79.4
rpl16_p5 AATAGGATCCCTCCCCATCG TACAAACCATTGGGGCGAAA 55.0 45.0 58.0 58.0 2262-2433 bp 0.736842 77.1