Hotspot

psbE-petL

LSC · 66093-67502 · 1410 bp

Back to Daphne

Pi 0.0154
MarkerSeek score 69.50
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1410
variable_sites
120
pi
0.0154
unique_haplotype_count
17
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA AGCTGAGGTTATAGTTAAAGCCA 50.0 39.1 57.9 57.3 1255-1581 bp 1.0 60.5
psbE-petL_p2 CAACCCGCAATGAATAGGGA AGTCGTATCTTACTCAGACCA 50.0 42.9 57.9 55.3 1284-1610 bp 1.0 60.2
psbE-petL_p3 CAACCCGCAATGAATAGGGA GCTGAGGTTATAGTTAAAGCCAC 50.0 43.5 57.9 57.3 1254-1580 bp 1.0 60.1
psbE-petL_p4 AGAACGTTCTCCTGTGCTTC AGCTGAGGTTATAGTTAAAGCCA 50.0 39.1 57.8 57.3 1178-1504 bp 1.0 59.9
psbE-petL_p5 ACGAATAACCAACCCGCAAT AGCTGAGGTTATAGTTAAAGCCA 45.0 39.1 58.2 57.3 1264-1590 bp 1.0 59.5