Hotspot

accD-psaI

LSC · 60251-60982 · 732 bp

Back to Daphne

Pi 0.0278
MarkerSeek score 69.50
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
732
variable_sites
99
pi
0.0278
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 ACTCCAAATCAGGTAGAGCC GGGTAAGCTGTTGAGAGTTGT 50.0 47.6 56.6 57.9 660-791 bp 0.947368 69.8
accD-psaI_p2 AACTCCAAATCAGGTAGAGCC GGGTAAGCTGTTGAGAGTTGT 47.6 47.6 57.4 57.9 661-792 bp 0.947368 67.8
accD-psaI_p3 TGACTCCAACTCCAAATCAGG GGGTAAGCTGTTGAGAGTTGT 47.6 47.6 57.6 57.9 716-799 bp 0.894737 67.4
accD-psaI_p4 GACTCCAACTCCAAATCAGGT GGGTAAGCTGTTGAGAGTTGT 47.6 47.6 57.6 57.9 715-798 bp 0.894737 67.4
accD-psaI_p5 ACTCCAACTCCAAATCAGGT GGGTAAGCTGTTGAGAGTTGT 45.0 47.6 56.3 57.9 714-797 bp 0.894737 65.5