Hotspot

ycf3-trnS-GGA

LSC · 45725-46310 · 586 bp

Back to Daphne

Pi 0.0136
MarkerSeek score 67.60
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
586
variable_sites
36
pi
0.0136
unique_haplotype_count
15
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 CATTTATCCGCGATCTAGGCA TGAATTAGGTGAAGGTGCGG 47.6 50.0 58.0 57.9 709-1032 bp 1.0 78.4
ycf3-trnS-GGA_p2 CATTTATCCGCGATCTAGGCA TTCCAATGCTACGCCTTGAA 47.6 45.0 58.0 57.8 643-966 bp 1.0 78.0
ycf3-trnS-GGA_p3 CATTTATCCGCGATCTAGGCA TTAGGTGAAGGTGCGGAAAG 47.6 50.0 58.0 57.8 705-1028 bp 1.0 77.8
ycf3-trnS-GGA_p4 CATTTATCCGCGATCTAGGCA GTGCGGAAAGAGAGGGATTC 47.6 55.0 58.0 58.3 695-1018 bp 1.0 76.6
ycf3-trnS-GGA_p5 CATTTATCCGCGATCTAGGCA GAATTAGGTGAAGGTGCGGA 47.6 50.0 58.0 57.6 708-1031 bp 1.0 76.1