Hotspot

trnG-GCC-trnfM-CAU

LSC · 37577-37730 · 154 bp

Back to Daphne

Pi 0.0433
MarkerSeek score 72.80
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
154
variable_sites
23
pi
0.0433
unique_haplotype_count
13
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 TCTTTGCCAAGGAGAAGACG CCTTTCATCGCGGGATAGAG 50.0 55.0 57.8 57.9 153-291 bp 1.0 88.8
trnG-GCC-trnfM-CAU_p2 TCTTTGCCAAGGAGAAGACG TAGCTCGCAAGGCTCATAAC 50.0 50.0 57.8 57.8 125-263 bp 1.0 88.3
trnG-GCC-trnfM-CAU_p3 TCAAGAACGAACAATGCGGA CCTTTCATCGCGGGATAGAG 45.0 55.0 58.1 57.9 195-333 bp 1.0 88.2
trnG-GCC-trnfM-CAU_p4 TCAAGAACGAACAATGCGGA GTAGCTCGCAAGGCTCATAA 45.0 50.0 58.1 57.8 168-306 bp 1.0 87.6
trnG-GCC-trnfM-CAU_p5 TCAAGAACGAACAATGCGGA TAGCTCGCAAGGCTCATAAC 45.0 50.0 58.1 57.8 167-305 bp 1.0 87.6