Hotspot

trnT-GGU-psbD

LSC · 32384-33654 · 1271 bp

Back to Daphne

Pi 0.0139
MarkerSeek score 66.70
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1271
variable_sites
91
pi
0.0139
unique_haplotype_count
19
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GGCGTAAGTCATCGGTTCAA TTTACCAAGGGCTATAGTCA 50.0 40.0 58.3 53.0 1086-1359 bp 1.0 49.5
trnT-GGU-psbD_p2 GTGGTAGAGTAACGCCATGG TTTACCAAGGGCTATAGTCA 55.0 40.0 58.1 53.0 1109-1382 bp 1.0 49.4
trnT-GGU-psbD_p3 TCAGTGGTAGAGTAACGCCA TTTACCAAGGGCTATAGTCA 50.0 40.0 58.1 53.0 1112-1385 bp 1.0 49.4
trnT-GGU-psbD_p4 TGGCCCTTTTAACTCAGTGG TTTACCAAGGGCTATAGTCA 50.0 40.0 57.7 53.0 1125-1398 bp 1.0 49.4
trnT-GGU-psbD_p5 GGCCCTTTTAACTCAGTGGT TTTACCAAGGGCTATAGTCA 50.0 40.0 57.7 53.0 1124-1397 bp 1.0 49.4