Hotspot

psbM-trnD-GUC

LSC · 30265-31013 · 749 bp

Back to Daphne

Pi 0.0201
MarkerSeek score 74.10
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
749
variable_sites
75
pi
0.0201
unique_haplotype_count
18
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 ATCAACAGCGCAGTAGCAAT AGTTCAACTGGTCAGAGCAC 45.0 50.0 58.3 57.8 604-878 bp 1.0 87.3
psbM-trnD-GUC_p2 AATTGCCTTGGCTGACTGTT AGTTCAACTGGTCAGAGCAC 45.0 50.0 58.3 57.8 663-937 bp 1.0 86.5
psbM-trnD-GUC_p3 ATTGCCTTGGCTGACTGTTT AGTTCAACTGGTCAGAGCAC 45.0 50.0 58.3 57.8 662-936 bp 1.0 86.5
psbM-trnD-GUC_p4 TCAACAGCGCAGTAGCAATA AGTTCAACTGGTCAGAGCAC 45.0 50.0 57.6 57.8 603-877 bp 1.0 86.3
psbM-trnD-GUC_p5 AATCAACAGCGCAGTAGCAA AGTTCAACTGGTCAGAGCAC 45.0 50.0 58.5 57.8 605-879 bp 1.0 85.5