Hotspot

trnK-UUU-rps16

LSC · 4344-5029 · 686 bp

Back to Daphne

Pi 0.0144
MarkerSeek score 67.60
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
686
variable_sites
69
pi
0.0144
unique_haplotype_count
16
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT TTTAAAGAAGGCGGGGGTTT 50.0 45.0 58.2 57.6 692-777 bp 0.947368 82.4
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT TTTTAAAGAAGGCGGGGGTT 50.0 45.0 58.2 57.6 693-778 bp 0.947368 82.4
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT TTTAAAGAAGGCGGGGGTTT 50.0 45.0 58.2 57.6 691-776 bp 0.947368 82.4
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TTTTAAAGAAGGCGGGGGTT 50.0 45.0 58.2 57.6 692-777 bp 0.947368 82.4
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT CGGGGGTTTACGGAACTTAA 50.0 50.0 58.2 57.5 680-765 bp 0.947368 81.8