Hotspot

rpl16

LSC · 40650-42325 · 1676 bp

Back to Danxiaorchis

Pi 0.0036
MarkerSeek score 60.90
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1676
variable_sites
6
pi
0.0036
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 AAAGAAATCCGTGGCTGTTT AACGAACAATTATATAAGGTTGAA 40.0 25.0 56.1 52.8 1807-1808 bp 1.0 40.6
rpl16_p2 AAGAAATCCGTGGCTGTTTT AAACGAACAATTATATAAGGTTGA 40.0 25.0 56.1 52.8 1807-1808 bp 1.0 40.6
rpl16_p3 AAGAAATCCGTGGCTGTTTT AACGAACAATTATATAAGGTTGAA 40.0 25.0 56.1 52.8 1806-1807 bp 1.0 40.6
rpl16_p4 AGAAATCCGTGGCTGTTTTT AAACGAACAATTATATAAGGTTGA 40.0 25.0 56.1 52.8 1806-1807 bp 1.0 40.6
rpl16_p5 AGAAATCCGTGGCTGTTTTT AACGAACAATTATATAAGGTTGAA 40.0 25.0 56.1 52.8 1805-1806 bp 1.0 40.6