Hotspot

accD

LSC · 26066-27547 · 1482 bp

Back to Danxiaorchis

Pi 0.00205

MarkerSeek score 61.40

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1482
variable_sites: 3
pi: 0.00205
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	ATAGGGGGCAGAAAGAATCT	CCCAAAGAAAACACCATCTTCT	45.0	40.9	55.6	56.8	1604-1622 bp	1.0	41.6
accD_p2	AATAGGGGGCAGAAAGAATCT	CCCAAAGAAAACACCATCTTCT	42.9	40.9	56.4	56.8	1605-1623 bp	1.0	41.6
accD_p3	ATAGGGGGCAGAAAGAATCT	CCCAAAGAAAACACCATCTTCTT	45.0	39.1	55.6	57.5	1604-1622 bp	1.0	41.6
accD_p4	AAATAGGGGGCAGAAAGAATCT	CCCAAAGAAAACACCATCTTCT	40.9	40.9	57.1	56.8	1606-1624 bp	1.0	41.6
accD_p5	ATAGGGGGCAGAAAGAATCT	CCCAAAGAAAACACCATCTTC	45.0	42.9	55.6	55.3	1604-1622 bp	1.0	41.6