Hotspot

ycf1-rpl32

SSC · 71497-73145 · 1649 bp

Back to Danxiaorchis

Pi 0.00321

MarkerSeek score 54.70

Primer available yes

Label ycf1-rpl32

Feature Metadata

label_name: ycf1-rpl32
feature_type: igs
region: SSC
length_bp: 1649
variable_sites: 3
pi: 0.00321
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.57
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rpl32_p1	AACAGCAGCCGTTTCAGTAT	TTCACCGGACAGTCAACAAG	45.0	50.0	57.8	58.1	1047-1760 bp	1.0	82.3
ycf1-rpl32_p2	ATAACAGCAGCCGTTTCAGT	TTCACCGGACAGTCAACAAG	45.0	50.0	57.8	58.1	1049-1762 bp	1.0	82.3
ycf1-rpl32_p3	CCAATAACAGCAGCCGTTTC	TTCACCGGACAGTCAACAAG	50.0	50.0	57.7	58.1	1052-1765 bp	1.0	81.7
ycf1-rpl32_p4	AGCAGCCGTTTCAGTATGTC	TTCACCGGACAGTCAACAAG	50.0	50.0	58.3	58.1	1044-1757 bp	1.0	81.6
ycf1-rpl32_p5	ACCAATAACAGCAGCCGTTT	TTCACCGGACAGTCAACAAG	45.0	50.0	58.4	58.1	1053-1766 bp	1.0	80.7