Hotspot

trnV-GAC-rrn16S

SSC · 58855-59067 · 213 bp

Back to Danxiaorchis

Pi 0.0142

MarkerSeek score 61.10

Primer available yes

Label trnV-GAC-rrn16S

Feature Metadata

label_name: trnV-GAC-rrn16S
feature_type: igs
region: SSC
length_bp: 213
variable_sites: 3
pi: 0.0142
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-GAC-rrn16S_p1	ATTTCTCGATGGGACTGGGA	TCCGACTCGCATGTGTTAAG	50.0	50.0	58.1	58.0	371-372 bp	1.0	79.8
trnV-GAC-rrn16S_p2	ATTTCTCGATGGGACTGGGA	CCGGGATCAAACTCTCCATG	50.0	55.0	58.1	58.0	327-328 bp	1.0	79.7
trnV-GAC-rrn16S_p3	TTTCTCGATGGGACTGGGAA	TCCGACTCGCATGTGTTAAG	50.0	50.0	58.3	58.0	370-371 bp	1.0	78.0
trnV-GAC-rrn16S_p4	TTTCTCGATGGGACTGGGAA	CCGGGATCAAACTCTCCATG	50.0	55.0	58.3	58.0	326-327 bp	1.0	77.9
trnV-GAC-rrn16S_p5	ATTTCTCGATGGGACTGGGA	GCAGGTTCTTACGCGTTACT	50.0	50.0	58.1	58.3	422-423 bp	1.0	77.4