Hotspot

rps7-rps12

SSC · 56517-56574 · 58 bp

Back to Danxiaorchis

Pi 0.0172

MarkerSeek score 62.40

Primer available yes

Label rps7-rps12

Feature Metadata

label_name: rps7-rps12
feature_type: igs
region: SSC
length_bp: 58
variable_sites: 1
pi: 0.0172
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps7-rps12_p1	TTCTTCTGCAGTACCTCGAC	CTGTGGAAAGCCGTATTCGA	50.0	50.0	57.0	58.0	190 bp	1.0	72.3
rps7-rps12_p2	TTCTTCTGCAGTACCTCGAC	TCTGTGGAAAGCCGTATTCG	50.0	50.0	57.0	58.0	191 bp	1.0	72.3
rps7-rps12_p3	TCTTCTGCAGTACCTCGACG	CTGTGGAAAGCCGTATTCGA	55.0	50.0	59.2	58.0	189 bp	1.0	70.8
rps7-rps12_p4	TCTTCTGCAGTACCTCGACG	TCTGTGGAAAGCCGTATTCG	55.0	50.0	59.2	58.0	190 bp	1.0	70.8
rps7-rps12_p5	TTTCTTCTGCAGTACCTCGAC	CTGTGGAAAGCCGTATTCGA	47.6	50.0	57.7	58.0	191 bp	1.0	70.0